co cultures (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
94
Structured Review
Thermo Fisher
co cultures
Co Cultures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+saponin+solution/Saponin+permeating+solution%2C+0%2E5%25+w%2Fv+soln%2E+in+PBS/bio_rxiv__64898__2026__06__11__725753-66-3-20
Average 94 stars, based on 1 article reviews
Co Cultures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+saponin+solution/Saponin+permeating+solution%2C+0%2E5%25+w%2Fv+soln%2E+in+PBS/bio_rxiv__64898__2026__06__11__725753-66-3-20
Average 94 stars, based on 1 article reviews
co cultures - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Cryoelectron tomography of HIV-1 cell-cell transmission conjugates reveals a secluded environment for viral assembly and transfer"
Article Title: Cryoelectron tomography of HIV-1 cell-cell transmission conjugates reveals a secluded environment for viral assembly and transfer
Journal: bioRxiv
doi: 10.64898/2026.06.11.725753
Figure Legend Snippet: ( A ) Comparison NL4-3 Envelope and Δ Env sequences. Dotted box indicates sequence deletion and hashed box with red cross indicates lack of translation due to frameshift and introduction of a stop codon ( B ) The percentage of all (single-cell and conjugate) GFP+CTDR+ events at 3h or 24h co-cultures of uninfected SupT1 R5 cells mixed with either NL4-3 IRES-eGFP or Δ Env IRES-eGFP infected SupT1 R5 cells. ( C ) Representative imaging cytometry of single cells and conjugates present in NL4-3 IRES-eGFP and Δ Env IRES-eGFP co-cultures at 3h and 24h. ( D ) Breakdown of the distribution of single cell and conjugate GFP+CTDR+ events within the 3-hour or 24-hour co-cultures. Data from N=1 experiment that was performed in duplicate.
Techniques Used: Comparison, Sequencing, Single Cell, Infection, Imaging, Cytometry
Figure Legend Snippet: ( A ) Representative dot plots of multicell conjugates in 3h co-cultures between target CTDR+ SupT1 R5 cells mixed with either NL4-3 IRES-eGFP or ΔEnv IRES-eGFP infected SupT1 R5 cells. ( B ) The percentage of GFP+CTDR+ multicell conjugate events from initial 0-hour or 3-hour co-cultures. ( C ) Representative dot plots of single cells in 24-hour co-cultures of target CTDR+ SupT1 R5 cells mixed with either NL4-3 IRES-eGFP or ΔEnv IRES-eGFP+ infected SupT1 R5 cells ( D ) The percentage of GFP+CTDR+ single cell events from initial 0-hour or 24-hour co-cultures. Distinct shapes in B and D each represent distinct biological replicates (N = 3) and each individual shape a technical replicate. (E-H) Same as A-D but with co-culture of infected GFP+ primary CD4+ T lymphocytes and target CTDR+ primary CD4+ T lymphocytes. Shapes in F and H represent distinct primary cell donors (N = 4). All values are normalized to initial GFP+ infection levels prior to co-culture. Values below x-axis indicate the mean and standard deviation for each category. Black bar represents the mean and the gray box represents ±1 standard deviation. P-values calculated using either two-tailed unpaired t- test ( B and D ) or two-tailed paired t- test ( F and H ).
Techniques Used: Infection, Single Cell, Co-Culture Assay, Standard Deviation, Two Tailed Test
Figure Legend Snippet: ( A , D ) Quantification of GFP+CTDR+ multicell conjugates events in 3-hour co-cultures of target CTDR+ SupT1 R5 and NL4-3 IRES-eGFP infected SupT1 R5 cells treated with varying concentrations of soluble CD4 (sCD4, A ) or anti-CD4 (SIM.2, D ). ( B and E ) Quantification of GFP+CTDR+ single cells in 24-hour co-cultures treated with varying concentrations of sCD4 ( B ) or SIM.2 ( E ). ( C and F ) Quantification of GFP+ SupT1 R5 after cell-free NL4-3 IRES-eGFP virus infection treated with varying concentrations of sCD4 ( C ) or SIM.2 ( F ). Data are derived from distinct biological replicates (N = 2), where colors represent distinct biological and each dot a technical replicate. All values are normalized to untreated controls and initial GFP+ infection levels prior to co-culture (if applicable). Values below x-axis indicate the mean and standard deviation for each category. Black bar represents the mean and the gray box represents ±1 standard deviation. P-values calculated using one-way ANOVA with a Tukey’s test for multiple comparisons.
Techniques Used: Infection, Virus, Derivative Assay, Co-Culture Assay, Standard Deviation
Related Articles
other:Article Title: Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein. Article Snippet: To propagate within a eukaryotic cell, pathogenic bacteria hijack and remodulate host cell functions.. The Gram-negative obligate intracellular Chlamydiaceae, which pose a serious threat to human and animal health, attach to host cells and inject effector proteins that reprogram host cell machineries.. Members of the conserved chlamydial TarP family have been characterized as major early effectors that bind to and remodel the host actin cytoskeleton.We now describe a new function for theChlamydia pneumoniae TarPmember CPn0572, namely the ability to bind and alter the microtubule cytoskeleton. Staining:Article Title: A Novel Virus Carrier State to Evaluate Immunotherapeutic Regimens: Regulatory T Cells Modulate the Pathogenicity of Anti-viral Memory Cells Article Snippet: .. Cells were then washed twice with a PBS-saponin solution and stained with Article Title: Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein Article Snippet: For permeabilization, 2% saponin (Merck KGaA, Germany) in PBS was used for 20 min at RT. .. Staining of MTs was performed with anti-α-tubulin antibody (Origene Technologies, Inc., Rockville, MD, USA; #BM753S; 1:150), acetylated MTs with Incubation:Article Title: Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation Article Snippet: .. Then, cells were successively incubated with primary and secondary antibodies in the same Article Title: Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell response Article Snippet: .. Then, cells were successively incubated with primary and secondary antibodies in the same |